Santanu Dutta
Articles written in Journal of Biosciences
Volume 6 Issue 6 December 1984 pp 817-821
Influence of formamide on the thermal stability of DNA
Chanchal Sadhu Santanu Dutta K P Gopinathan
The utility of formamide in the denaturation and renaturation of DNA has been examined. The melting temperature of duplex DNA is lowered by 0·6°C per per cent formamide. The depression of melting temperature is independent of the GC content. Formamide also increases the width of the thermal transition. Upto 30%, it does not affect the rate of DNA reassociation
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