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    • Volume 43 Issue 3 July 2018 pp 447-454 Article

      Single-molecule photobleaching: Instrumentation and applications

      SIMLI DEY SUDIPTA MAITI

      More Details Abstract Fulltext PDF

      Single-molecule photobleaching (smPB) technique is a powerful tool for characterizing molecular assemblies. It canprovide a direct measure of the number of monomers constituting a given oligomeric particle and generate the oligomer sizedistribution in a specimen. A major current application of this technique is in understanding protein aggregation, which islinked to many incurable diseases. Quantitative measurement of the size distribution of an aggregating protein in aphysiological solution remains a difficult task, since techniques such as dynamic light scattering or fluorescence correlationspectroscopy (FCS) can provide an average size, but cannot accurately resolve the underlying size distribution. Here wedescribe the smPB method as implemented on a home-built total internal reflection fluorescence microscope (TIRF). Wefirst describe the construction of a TIRF microscope, and then demonstrate the power of smPB by characterizing a solutionof Amylin (hIAPP) oligomers, a 37-residue peptide whose aggregation is associated with Type II diabetes. We compare ourresults with FCS data obtained from the same specimen, and discuss the advantages and disadvantages of the twotechniques.

  • Journal of Biosciences | News

      Forthcoming Special issue.


    • To trigger further research on plant mitochondria, the Journal of Biosciences is bringing out a special issue titled "Plant Mitochondria: Properties and Interactions with Other Organelles".


      Plant mitochondria are quite distinct and have unique features, such as a cyanide-insensitive alternate pathway. They also interact with chloroplasts to optimize photosynthetic carbon assimilation.


      Submissions are welcome until 30 July 2023. The contributions can be original articles, short communications, reviews, or mini-reviews on any topic related to plant mitochondria.


      Authors can submit their articles online at https://www.editorialmanager.com/jbsc/default2.aspx

      Posted on April 12, 2023
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