Articles written in Journal of Biosciences
Volume 42 Issue 1 March 2017 pp 131-138 Article
The aim of this study was to investigate whether neonatal maternal separation (MS) – chronic stress experience inearly life – affects the anorectic efficacy of leptin in the offspring at adolescence. Sprague–Dawley pups wereseparated from the dam daily for 3 h during postnatal day 1–14 or left undisturbed as non-handled controls (NH).NH and MS male pups received an intraperitoneal leptin (100 μg/kg) or saline on postnatal day (PND) 28, and thenfood intake and body weight gain were recorded. The hypothalamic levels of leptin-signalling-related genes,phosphorylated signal transducer and activator of transcription-3 (pSTAT3) and protein-tyrosine phosphatase 1B(PTP1B) were examined at 40 min after a single injection of leptin on PND 39 by immunohistochemistry and Westernblot analysis. Leptin-induced suppressions in food intake and weight gain was observed in NH pups, but not in MS.Leptin increased pSTAT3 in the hypothalamic arcuate nucleus of NH pups, but not of MS. Interestingly, basal levelsof the hypothalamic PTP1B and pSTAT3 were increased in MS pups compared with NH controls. The results suggestthat neonatal MS experience may blunt the anorectic efficacy of leptin later in life, possibly in relation with increasedexpressions of PTP1B and/or pSTAT3 in the hypothalamus.
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