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SAMRAT MUKHOPADHYAY
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Volume 43 Issue 3 July 2018 pp 455-462 Article
DEBAPRIYA DAS SAMRAT MUKHOPADHYAY
Intrinsically disordered proteins (IDPs) do not autonomously adopt a stable unique 3D structure and exist as an ensemble ofrapidly interconverting structures. They are characterized by significant conformational plasticity and are associated withseveral biological functions and dysfunctions. The rapid conformational fluctuation is governed by the backbone segmentaldynamics arising due to the dihedral angle fluctuation on the Ramachandran ϕ–Ψ conformational space. We discovered thatthe intrinsic backbone torsional mobility can be monitored by a sensitive fluorescence readout, namely fluorescencedepolarization kinetics, of tryptophan in an archetypal IDP such as α-synuclein. This methodology allows us to map thesite-specific torsional mobility in the dihedral space within picosecond-nanosecond time range at a low protein concentrationunder the native condition. The characteristic timescale of * 1.4 ns, independent of residue position, representscollective torsional dynamics of dihedral angles (ϕ and Ψ) of several residues from tryptophan and is independent of overallglobal tumbling of the protein. We believe that fluorescence depolarization kinetics methodology will find broad applicationto study both short-range and long-range correlated motions, internal friction, binding-induced folding, disorder-to-ordertransition, misfolding and aggregation of IDPs.
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Journal of Biosciences
Volume 48, 2023
All articles
Continuous Article Publishing mode
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Journal of Biosciences | News
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To trigger further research on plant mitochondria, the Journal of Biosciences is bringing out a special issue titled "Plant Mitochondria: Properties and Interactions with Other Organelles".
Plant mitochondria are quite distinct and have unique features, such as a cyanide-insensitive alternate pathway. They also interact with chloroplasts to optimize photosynthetic carbon assimilation.
Submissions are welcome until 30 July 2023. The contributions can be original articles, short communications, reviews, or mini-reviews on any topic related to plant mitochondria.
Authors can submit their articles online at https://www.editorialmanager.com/jbsc/default2.aspx
Posted on April 12, 2023< -
Editorial Note on Continuous Article Publication
Posted on July 25, 2019Click here for Editorial Note on CAP Mode
Forthcoming Special issue.
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