• SAI BHARADWAJA

      Articles written in Journal of Biosciences

    • An in vitro mechanistic approach towards understanding the distinct pathways regulating insulin resistance and adipogenesis by apocynin

      SAI BHARADWAJA PRAVEEN KUMAR ISSAC JOCELYN CLETA RAKESH JEGANATHAN SRI SNEHAA CHANDRAKUMAR SUJATHA SUNDARESAN

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      Adipogenesis is a cascade of processes that entail the differentiation of fibroblasts into mature adipocytes,which results in the accumulation of triglycerides in the adipose cells due to high dietary supplements. Thisphysiological condition increases the risk of type 2 diabetes. Apocynin (4-hydroxy-3-methoxyacetophenone),an organic compound from the root extracts of the medicinal herb Picrorhiza kurroa, has been used in variousexperimental studies. The current study focuses on deciphering the cellular and molecular mechanismsinterlinking obesity and diabetes by validating the various key targets involved in insulin signaling andadipogenesis. Apocynin exhibited enhanced glucose uptake and decreased lipid accumulation in the adipocytes.Furthermore, the expression of molecular markers involved in the insulin signaling pathway, such asIRTK, IRS-1, PI3K, GLUT-4, and the adipogenic pathway, such as PPAR-alpha, adiponectin, C/EBP-alpha andSREBP1C, by qPCR supported our hypothesis largely. Apocynin mimicked insulin in the insulin-signalingpathway by showing equivalent gene expression. It ameliorated adipogenesis by downregulating the keymarkers in the adipogenic pathway. Corroborating the hypothesis that Apocynin is antihyperlipidemic innature, it reduced the expression of PPAR-alpha and adiponectin. These results substantiate that Apocynin exertsanti-diabetic and anti-adipogenic effects by regulating resistin and antioxidant enzyme levels in vitro.

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