Articles written in Journal of Biosciences
Volume 46 All articles Published: 13 March 2021 Article ID 0026 Article
Due to the broad-spectrum of antibiotic resistance, herein we investigated the possibility of using imipenemconjugatedsilver nanoparticles (IMP-AgNPs) against multidrug-resistant isolates of Pseudomonas aeruginosa.For this purpose, 200 clinical isolates were tested against different antibiotics to determine the antimicrobialsusceptibility. To identify blaVIM and blaIMP resistance genes, PCR was used. The synthesized AgNPs andconjugants were characterized using UV-vis spectroscopy, XRD, SEM, TEM, DLS, and FTIR. The stability,drug release kinetics, cytotoxicity, hemolytic and apoptotic effects of NPs were also investigated. MIC of theimipenem, AgNPs, and conjugants were evaluated versus P. aeruginosa isolates. Finally, the effects of theIMP-AgNPs to heal burn wounds in rats was evaluated. According to the results, about 68% of isolates showedresistance to imipenem (MIC >= 64 lg/ml to >=512 lg/ml). Analytical results verified the synthesis of AgNPsand IMP-AgNPs. A Dose-dependent decrease happened in terms of the MIC values of IMP-AgNPs were alsoaffected by the existence of resistant genes. Low cytotoxic was observed regarding AgNPs which lead toapoptosis. The histopathological results showed a considerable epithelization in treated groups with IMPAgNPs.Accordingly, IMP-AgNPs can be considered as a powerful antibacterial agent to treat the infectionscaused by multidrug-resistant P. aeruginosa.
Volume 46, 2021
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