Feeding of malathion induces accumulation of acetylcholine inPhilosamia ricini developing larvaevia inhibition of acetyl cholinesterase activity. The insecticide also causes depletion of all nutrients, loss in weight, under-development of silk glands resulting in reduced silk production, lack of oviposition and high mortality among the insects. Acetylcholine however, while fed during the same period of development, tones up their nutritional status, induces better growth, weight gain, improved silk production and oviposition and significantly lower mortality than in the control group of insects maintained on castor leaves. This improved status of insects has been attributed to choline, the insect vitamin released from acetylcholine. Acetylcholine has also been noted to protect the insects to some extent from the poisonous effect of malathion on exposure to it after a dose of acetylcholine during the preceding instar stadium