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RANA P SINGH
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Volume 43 Issue 2 June 2018 pp 249-261 Article
Lupeol induces S-phase arrest and mitochondria-mediated apoptosis in cervical cancer cells
NUPOOR PRASAD AKASH SABARWAL UMESH C S YADAV RANA P SINGH
Cervical cancer is fourth most common fatal cancer in women worldwide. Lupeol is a dietary triterpenoid and has shown itsanticancer efficacy against various cancer types with selectivity in targeting cancer cells. In the present study, anticancerefficacy and mechanism of action of a phytochemical, lupeol, in human cervical carcinoma (HeLa) cells has been examined.The anticancer efficacy of lupeol was assessed by trypan blue cell counting, annexin Vassay, cell cycle analysis, expressionof apoptotic proteins by RT-PCR and Western blotting and assessment of mitochondrial ROS generation by mitosox andmitotracker assays. Our results demonstrated that lupeol decreased cell proliferation and viability of HeLa cells significantly(p\0.001). Lupeol induced S-phase cell cycle arrest and also decreased the expression of S-phase Cyclins and CDKs andincreased the expression of cyclin-dependent kinase inhibitors, p21 at transcriptional and translational level. Further, lupeolinduced apoptosis and increased the expression of apoptosis markers such as cleaved PARP and Bax:Bcl-2 ratio. Furthermore,mitosox and mitotracker dye incubation followed by FACS analysis showed an increase in mitochondrialsuperoxide generation and reduction in healthy mitochondrial mass. These results suggest that lupeol could be an effectivechemotherapeutic agent against cervical carcinoma due to its growth inhibitory activity through induction of S-phase cellcycle arrest and apoptosis.
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Journal of Biosciences
Volume 48, 2023
All articles
Continuous Article Publishing mode
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Journal of Biosciences | News
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To trigger further research on plant mitochondria, the Journal of Biosciences is bringing out a special issue titled "Plant Mitochondria: Properties and Interactions with Other Organelles".
Plant mitochondria are quite distinct and have unique features, such as a cyanide-insensitive alternate pathway. They also interact with chloroplasts to optimize photosynthetic carbon assimilation.
Submissions are welcome until 30 July 2023. The contributions can be original articles, short communications, reviews, or mini-reviews on any topic related to plant mitochondria.
Authors can submit their articles online at https://www.editorialmanager.com/jbsc/default2.aspx
Posted on April 12, 2023< -
Editorial Note on Continuous Article Publication
Posted on July 25, 2019Click here for Editorial Note on CAP Mode
Forthcoming Special issue.
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