• RAJESH SRINIVASAN

      Articles written in Journal of Biosciences

    • Rapid diagnosis of Leishmania infection with a portable loop-mediated isothermal amplification device

      MADHU PURI HARSIMRAN KAUR BRAR NIMISHA MITTAL EVANKA MADAN RAJESH SRINIVASAN KAPIL RAWAT SRIJA MOULIK MITALI CHATTERJEE SAI SIVA GORTHI ROHINI MUTHUSWAMI RENTALA MADHUBALA

      More Details Abstract Fulltext PDF

      L. donovani is an intracellular protozoan parasite, that causes visceral leishmaniasis (VL), and consequently,post-kala azar dermal leishmaniasis (PKDL). Diagnosis and treatment of leishmaniasis is crucial for decreasingits transmission. Various diagnostic techniques like microscopy, enzyme-linked immunosorbent assays(ELISA) and PCR-based methods are used to detect leishmaniasis infection. More recently, loop-mediatedisothermal amplification (LAMP) assay has emerged as an ideal diagnostic measure for leishmaniasis, primarilydue to its accuracy, speed and simplicity. However, point-of-care diagnosis is still not been tested withthe LAMP assay. We have developed a portable LAMP device for the monitoring of Leishmania infection. TheLAMP assay performed using our device can detect and amplify as little as 100 femtograms of L. donovaniDNA. In a preliminary study, we have shown that the device can also amplify L. donovani DNA present in VLand PKDL patient samples with high sensitivity (100%), specificity (98%) and accuracy (99%), and can beused both for diagnostic and prognostic analysis. To our knowledge, this is the first report to describe thedevelopment and application of a portable LAMP device which has the potential to evolve as a point-of-carediagnostic and prognostic tool for Leishmania infections in future.

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