Articles written in Journal of Biosciences
Volume 21 Issue 2 April 1996 pp 207-219
Volume 23 Issue 4 October 1998 pp 377-386
Volume 24 Issue 3 September 1999 pp 295-299 Articles
DNA typing using genome derived cloned probes may be conducted for ascertaining genetic affinities of closely related species. We analysed gaur
Volume 36 Issue 3 August 2011 pp 499-504 Articles
The enormous advances in our understanding of the progression of diseases at the molecular level have been supplemented by the new field of ‘molecular imaging’, which provides for
optical bioluminescence and fluorescence imaging techniques,
radionuclide-based positron emission tomography (PET) and single photon emitted computed tomography (SPECT),
X-ray-based computed tomography (CT),
magnetic resonance imaging (MRI) and
ultrasound imaging (US).
Functional molecular imaging requires an imaging probe that is specific for a given molecular event. In preclinical imaging, involving small animal models, the imaging probe could be an element of a direct (`direct imaging’) or an indirect (`indirect imaging’) event. Reporter genes are essential for indirect imaging and provide a general integrated platform for many different applications. Applications of multimodality imaging using combinations of bioluminescent, fluorescent and PET reporter genes in unified fusion vectors developed by us for recording events from single live cells to whole animals with high sensitivity and accurate quantification are discussed. Such approaches have immense potential to track progression of metastasis, immune cell trafficking, stem cell therapy, transgenic animals and even molecular interactions in living subjects.