• Parul Varma

      Articles written in Journal of Biosciences

    • Dynamics of nuclear matrix proteome during embryonic development in Drosophila melanogaster

      Parul Varma Rakesh K Mishra

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      Embryonic development is a complex and dynamic process that involves spatiotemporal expression of genes in a highly coordinated manner. Multiple levels of nuclear architecture maintain the fidelity of gene expression programme. One of the components of nuclear architecture, which is believed to play an important role in regulation of gene expression, is the nuclear matrix (NuMat). Many studies over the past few years have tried to analyse the components of this non-chromatin scaffolding of the nucleus and have provided evidences of its structural and functional complexity. However, the relationship of NuMat with the process of embryonic development still remains poorly understood. Here, we report a comparative analysis of the NuMat proteomes of early and late stage Drosophila melanogaster embryos and show that 65% of the NuMat proteome is dynamic during development. Our study establishes links between the dynamics of nuclear architecture and embryonic development and provides tools to further understand the process such as cellular differentiation in the context of higher-order nuclear organization.

    • Little imaginal discs, a Trithorax group member, is a constituent of nuclear matrix of Drosophila melanogaster embryos


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      Nuclear Matrix (NuMat) is the structural and functional framework of the nucleus. It has been shown that attachment ofchromatin to NuMat brings significant regulation of the transcriptional activity of particular genes; however, key componentsof NuMat involved in this process remain elusive. We have identified Lid (Little imaginal discs) as one of thecomponents of NuMat. It belongs to the TrxG group of proteins involved in activation of important developmental genes.However, unlike other activator proteins of TrxG, Lid is a Jumonji protein involved in H3K4me3 demethylation. Here, wereport the association of Lid and its various domains with NuMat which implicates its structural role in chromatinorganization and epigenetic basis of cellular memory. We have found that both N and C terminal regions of this protein arecapable of associating with NuMat. We have further mapped the association of individual domains and found that, PHD,ARID and JmjC domains can associate with NuMat individually. Moreover, deletion of N-terminal PHD finger does notalter Lid’s NuMat association implying that although it is sufficient, yet, it is not necessary for Lid’s structural role inNuMat. Based on our findings, we hypothesize that C terminal region of Lid which contains PHD fingers might beresponsible for its NuMat association via protein–DNA interactions. However, for the N terminal region harboring both aPHD and an ARID finger, Lid anchors to the NuMat via both protein-protein and protein-DNA interactions. The associationof JmjC domain with NuMat is the first report of the association of a demethylase domain with NuMat suggesting that Lid,a demethylase, being part of NuMat might be involved in regulating the chromatin dynamics via its NuMat association.

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