P D Gupta
Articles written in Journal of Biosciences
Volume 12 Issue 3 September 1987 pp 257-265
Conditions have been standardized to maintain rat vaginal epithelial cells
Volume 13 Issue 2 June 1988 pp 109-116
Rat vaginal epithelial layers from animals in different phases of the estrous cycle showed positive immunofluorescence when treated with either monoclonal antibody to intermediate filaments or immunoglobulin G fraction of antiserum raised against epidermal keratin filaments. During estrus, the intensity of fluorescence observed was maximum in the keratinized cellular layers. In estradiol-primed immature and ovariectomized rats the maximum fluorescence intensity was observed in the layers immediately lining the lumen. However, basal layers in ovariectomized rats also showed some fluorescence. Data presented in this communication indicate that the abundance of keratin filaments in vaginal epithelial cells can be modulated by altering the level of estradiol in the system.
Volume 13 Issue 4 December 1988 pp 401-408
Calcium-dependent transglutaminase activity was found to be present in vaginal homogenates from adult cycling rats. Treatment of immature or adult ovariectomized rats with oestradiol (0.1 μg/g body weight) resulted in 1.5–2-fold enhancement in the enzyme activity. Progesterone treatment (0.1 μg/g body weight) decreased the enzyme activity. Analysis of amino acids produced by proteolytic enzyme digestion of insoluble keratins from rat vaginal tissue indicated the presence of γ-glutamyl-ε-⥿sine dipeptide (4 μmol/g protein) in this protein. These results suggest that oestradiol acts on vaginal tissue and induces the activity of transglutaminase. This enzyme catalyses the formation of γ-glutamyl-ε-lysine crosslinks between keratin polypeptides and thus leads to kerartization of the tissue.
Volume 22 Issue 3 June 1997 pp 273-285