Salt stress is a major abiotic factor that affects the growth and yield of crops. The present study was carried outto assess the salt tolerance among the Arka Samrat, Arka Rakshak, YVU-1, S-22, YVU-2, and PKM-OPtomato germplasms using principal component analysis (PCA). Different salt (NaCl) concentrations likecontrol, 0.04 M, 0.12 M, and 0.20 M were selected in order to classify them into sensitive and tolerant tomatogermplasms based on 13 parameters. A significant variation was observed among the selected tomato germplasmstowards salinity tolerance at the seedling stage. Shoot length, root length, fresh weight, and dry weightparameters of the seedlings were decreased linearly with an increase in the external NaCl concentration.Salinization of plants has shown to reduce K+ content and increase in the Na+ accumulation, Ca2+, andCatalase activity. Salt stress also increased electrolyte leakage and reduced relative water content of allgermplasms. The maximum parameters were less affected in Arka Rakshak and Arka Samrat compared to theremaining germplasms at higher salt stress. The PCA analysis of 13 morphological and physiological variablesindicated that Arka Rakshak and Arka Samrat germplasms were salt-tolerant and PKM-OP was susceptible.Thus PCA analysis results are useful for the identification of resistance and sensitive germplasms at theseedling stage.