NOOR AHMED MUBARAK BEN ZAKAR
Articles written in Journal of Biosciences
Volume 43 Issue 5 December 2018 pp 897-909 Article
The outcomes of compressed spinal cord injury (CSCI) necessitate radical treatment. The therapeutic potential of neuroectodermalstem cells (NESCs) in a rat model of CSCI in acute and subacute stages was assessed. White Wistar rat weredivided into control, sham-operated, CSCI untreated model, CSCI grafted with NESCs at 1 day after CSCI, and at 7 daysafter CSCI. Primary NESC cultures were prepared from brains of embryonic day 10 (E10) mice embryos. NESCs weretransplanted at the site of injury using a Hamilton syringe. Locomotor functional assessment, routine histopathology,immunostaining for (GFAP), and ultrastructure techniques for evaluating the CSI were conducted. In CSCI, areas ofhemorrhage, cavitation, reactive astrocytosis, upregulated GFAP expression of immunostained areas, degeneration of theaxoplasm and demyelination were observed. One day after grafting with NESCs, a decrease in astrocyte reaction andpathological features, quantitative and qualitative enhancement of remyelination and improved locomotor activity wereobserved. Treatment with NESCs at 7 days after CSCI did not mitigatethe reactive astrocytosis and glial scar formation thathindered the ability of the NESCs to enhance remyelination of axons. In conclusion, the microenvironment and time ofNESCs transplantation affect activity of astrocytes and remyelination of axons.
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