Articles written in Journal of Biosciences
Volume 7 Issue 2 March 1985 pp 115-122
Extra-gonadal secretion of an inhibin-like substance in the laboratory albino rat
Kusum Kharbanda MeenaKumari S Mohapatra S Duraiswami
Acetone powder preparations of ventral prostates of adult albino rat exhibit an inhibin-like activity.
Volume 10 Issue 3 September 1986 pp 413-422
A method for the isolation of intact Sertoli cell-germ cell associations from rat seminiferous tubules and their further partition into Sertol cell and germ cell fractions
A technique is described for obtaining a Sertoli cell-enriched and a germ cell-enriched fraction from immature rat testes. Sertoli cell-germ cell associations were obtained by incubating washed seminiferous tubule fragments with Collagenase and Pancreatin. They were then manually dissociated into a suspension comprising Sertoli cells as well as the various germ cell types characteristic for a given day of ontogeny. Fractionation into a Sertoli cell-enriched fraction and a germ cell-enriched fraction was effected by centrifugation following layering over a stepwise gradient of Ficoll-400. While the time-span compares favourably with other procedures reported in the literature, it is believed this is the first time a method is described that enables the simultaneous recovery of both the Sertoli cells and the germ cells.
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