• MOHAMMAD HOSSEIN GHAHREMANI

      Articles written in Journal of Biosciences

    • Induction of morphological and functional differentiation of human neuroblastoma cells by miR-124

      SAMANEH SHARIF MOHAMMAD HOSSEIN GHAHREMANI MASOUD SOLEIMANI

      More Details Abstract Fulltext PDF

      Neuroblastoma is the most common extracranial solid tumour in children, and differentiation is considered its mostappropriate therapy. In this work, we studied effects of miR-124 overexpression on differentiation in M17 cell line as amodel of neuroblastoma cancer. Influence of miR-124 overexpression on differentiation in M17 cells was studied. M17cells were infected with lentivirus that contained miR-124 precursor sequence and followed for 2 weeks to differentiate.Ectopic expression of miR-124 in M17 cells changed the shape of spherical undifferentiated cells to cells with extendedneurites that formed neuronal networks. Overexpression of MiR-124 respectively increased the expression level of markersof beta-Tubulin III, MAP2, SYN, NF-M and Nestin by 16-, 5-, 4-, 2.3- and 2-folds at the messenger RNA level. MiR-124overexpression also increased the protein levels of beta-Tubulin III and MAP2. Moreover, exogenous expression of miR-124significantly increased the intracellular calcium in differentiated M17 cells. Since miR-124 is naturally expressed inneuronal cells and is downregulated in neuroblastoma cancer cells, differentiation with this type of microRNA can be anovel treatment for neuroblastoma cancer.

  • Journal of Biosciences | News

    • Editorial Note on Continuous Article Publication

      Posted on July 25, 2019

      Click here for Editorial Note on CAP Mode

© 2017-2019 Indian Academy of Sciences, Bengaluru.