MOHAMMAD HOSSEIN GHAHREMANI
Articles written in Journal of Biosciences
Volume 42 Issue 4 December 2017 pp 555-563 Article
Neuroblastoma is the most common extracranial solid tumour in children, and differentiation is considered its mostappropriate therapy. In this work, we studied effects of miR-124 overexpression on differentiation in M17 cell line as amodel of neuroblastoma cancer. Influence of miR-124 overexpression on differentiation in M17 cells was studied. M17cells were infected with lentivirus that contained miR-124 precursor sequence and followed for 2 weeks to differentiate.Ectopic expression of miR-124 in M17 cells changed the shape of spherical undifferentiated cells to cells with extendedneurites that formed neuronal networks. Overexpression of MiR-124 respectively increased the expression level of markersof beta-Tubulin III, MAP2, SYN, NF-M and Nestin by 16-, 5-, 4-, 2.3- and 2-folds at the messenger RNA level. MiR-124overexpression also increased the protein levels of beta-Tubulin III and MAP2. Moreover, exogenous expression of miR-124significantly increased the intracellular calcium in differentiated M17 cells. Since miR-124 is naturally expressed inneuronal cells and is downregulated in neuroblastoma cancer cells, differentiation with this type of microRNA can be anovel treatment for neuroblastoma cancer.
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