• MASAFUMI SAIJO

      Articles written in Journal of Biosciences

    • Xeroderma pigmentosum A homolog from Hydra partially complements DNA repair defect in human XPA-deficient cells

      ALISHA A GALANDE MASAFUMI SAIJO SAROJ S GHASKADBI SURENDRA GHASKADBI

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      Nucleotide excision repair (NER) pathway is a DNA repair mechanism that rectifies a wide spectrum of DNAlesions. Xeroderma pigmentosum group of proteins (XPA through XPG) orchestrate the NER pathway inhumans. We have earlier studied XPA homolog from Hydra (HyXPA) and found it to be similar to humanXPA. Here, we examined if HyXPA can functionally complement human XPA-deficient cells and reduce theirsensitivity to UV radiation. We found that HyXPAwas able to partially rescue XPA-deficient human cells fromUV by its binding to chromatin of UV-irradiated cells. However, HyXPA failed to bind replication protein A(RPA70), a key interacting partner of human XPA in NER pathway. This could be attributed to changes incertain amino acid residues that have occurred during evolution, leading to prevention of some interactionsbetween Hydra and human proteins.

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