Articles written in Journal of Biosciences
Volume 32 Issue 6 September 2007 pp 1119-1131 Articles
Productivity and biochemical properties of green tea in response to full-length and functional fragments of HpaG
Harpin proteins from plant pathogenic bacteria can stimulate hypersensitive cell death (HCD), drought tolerance, defence responses against pathogens and insects in plants, as well as enhance plant growth. Recently, we identified nine functional fragments of HpaGXooc, a harpin protein from
Volume 35 Issue 3 September 2010 pp 435-450 Articles
The harpin protein HrpNEa induces
Volume 44 Issue 1 March 2019 Article ID 0017 Article
Autophagy is a highly conserved intracellular degradation pathway in eukaryotic cells that responds to environmentalchanges. Genetic analyses have shown that more than 40 autophagy-related genes (ATG) are directly involved in thisprocess in fungi. In addition to Atg proteins, most vesicle transport regulators are also essential for each step of autophagy.The present study showed that one Endoplasmic Reticulum protein in Saccharomyces cerevisiae, Tip20, which controlsGolgi-to-ER retrograde transport, was also required for starvation-induced autophagy under high temperature stress. Intip20 conditional mutant yeast, the transport of Atg8 was impaired during starvation, resulting in multiple Atg8 punctadispersed outside the vacuole that could not be transported to the pre-autophagosomal structure/phagophore assembly site(PAS). Several Atg8 puncta were trapped in ER exit sites (ERES). Moreover, the GFP-Atg8 protease protection assayindicated that Tip20 functions before autophagosome closure. Furthermore, genetic studies showed that Tip20 functionsdownstream of Atg5 and upstream of Atg1, Atg9 and Atg14 in the autophagy pathway. The present data show that Tip20,as a vesicle transport regulator, has novel roles in autophagy.
Volume 44 Issue 4 September 2019 Article ID 0082 Article
Type-III (T3) effectors PthXo1 and AvrXa10 of Xanthomonas oryzae pv. oryzae are translocated into rice cells to inducevirulence and avirulence on susceptible- and resistant-rice varieties Nipponbare and IRBB10, respectively. The translocationneeds the bacterial T3 translocator Hpa1 and rice Oryza sativa plasma membrane protein OsPIP1;3. Here, weemployed the b-lactamase (BlaM) reporter system to observe PthXo1 and AvrXa10 translocation. The system wasestablished to monitor effectors of animal-pathogenic bacteria by quantifying the BlaM hydrolysis product [P] and fluorescenceresonance energy transfer (FRET) of the substrate. The feasibility of the BlaM reporter in rice protoplasts wasevaluated by three criteria. The first criterion indicated differences between both [P] and FRET levels among wild types andOsPIP1;3-overexpressing and OsPIP1;3-silenced lines of both Nipponbare and IRBB10. The second criterion indicateddifferences between [P] and FRET levels in the presence and absence of Hpa1. The last criterion elucidated the coincidenceof PthXo1 translocation with induced expression of the PthXo1 target gene in protoplasts of Nipponbare and the coincidenceof AvrXa10 translocation with induced expression of the AvrXa10 target gene in protoplasts of IRBB10. Theseresults provide an experimental avenue for real-time monitoring of bacterial T3 effector translocation into plant cells with apathological consequence.
Volume 44 | Issue 5
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