Articles written in Journal of Biosciences
Volume 24 Issue 4 December 1999 pp 409-411 Clipboard
Volume 27 Issue 5 September 2002 pp 479-487 Articles
We have identified the
Volume 39 Issue 1 March 2014 pp 3-11 Clipboard
Secretion is widespread in all eukaryotic cells: all of us experience this in the course of daily life – saliva, mucus, sweat, tears, bile juice, adrenalin, etc. – the list is extremely long. How does a cell manage to repeatedly spit out some stuff without losing the rest? The answer is: through regulated vesicle trafficking within the cell. The Nobel Prize in Physiology or Medicine 2013 was awarded to Drs Randy Schekman, James E Rothman and Thomas C Südhof for their ‘discoveries of machinery regulating vesicle traffic, a major transport system in our cells’. Dr Randy Schekman and his colleagues discovered a number of genes required for vesicle trafficking from the endoplasmic reticulum (ER) and Golgi; the James E Rothman group unravelled the protein machinery that allows vesicles to bud off from the membrane and fuse to their targets; and Dr Thomas C Südhof along with his colleagues revealed how calcium ions could instruct vesicles to fuse and discharge their contents with precision. These enabled the biotechnology industry to produce a variety of pharmaceutical and industrial products like insulin and hepatitis B vaccines, in a cost-efficient manner, using yeast and tissue cultured cells.
Volume 41 Issue 2 June 2016 pp 219-227 Article
Odorant receptors (OR) heterodimerizes with the OR co-receptor (Orco), forming specific odorant-gated cation channels, which are key to odor reception at the olfactory sensory neurons (OSN). Mammalian ORs are expressed in many other tissues, including testis. However, their biological implications are yet to be fully ascertained. In the mosquito, Orco is localized along the sperm tail and is indicated to maintain fidelity. Here, we show that orco expresses in Drosophila testis. The levels are higher in the somatic cyst cells. The orco-null mutants are perfectly fertile at 25°C. At 28°C, the coiled spermatid bundles are severely disrupted. The loss of Orco also disrupts the actin cap, which forms inside the head cyst cell at the rostral ends of the spermatid nuclei after coiling, and plays a key role in preventing the abnormal release of spermatids from the cyst enclosure. Both the defects are rescued by the somatic cyst cell-specific expression of the UAS-orco transgene. These results highlight a novel role of Orco in the somatic tissue during sperm release.
Volume 42 Issue 2 June 2017 pp 285-297 Article
Spermatogenesis, involving multiple transit amplification divisions and meiosis, occurs within an enclosure formed bytwo somatic cells. As the cohort of germline cells divide and grow, the surface areas of the somatic cells expandmaintaining a tight encapsulation throughout the developmental period. Correlation between the somatic cell growthand germline development is unclear. Here, we report standardization of a quantitative assay developed for estimatingthe somatic roles of target molecules on germline division and differentiation in Drosophila testis. Using the assay, westudied the somatic roles of phosphatidylinositol-3-kinase (PI3K). It revealed that the expression of PI3KDN is likelyto facilitate the early germline development at all stages, and an increase in the somatic PI3K activity during the earlystages delays the transition to spermatocyte stage. Together, these results suggest that somatic cell growth plays animportant role in regulating the rate of germline development.