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KAPIL RAWAT
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Volume 46 All articles Published: 23 September 2020 Article ID 0092 Article
MADHU PURI HARSIMRAN KAUR BRAR NIMISHA MITTAL EVANKA MADAN RAJESH SRINIVASAN KAPIL RAWAT SRIJA MOULIK MITALI CHATTERJEE SAI SIVA GORTHI ROHINI MUTHUSWAMI RENTALA MADHUBALA
L. donovani is an intracellular protozoan parasite, that causes visceral leishmaniasis (VL), and consequently,post-kala azar dermal leishmaniasis (PKDL). Diagnosis and treatment of leishmaniasis is crucial for decreasingits transmission. Various diagnostic techniques like microscopy, enzyme-linked immunosorbent assays(ELISA) and PCR-based methods are used to detect leishmaniasis infection. More recently, loop-mediatedisothermal amplification (LAMP) assay has emerged as an ideal diagnostic measure for leishmaniasis, primarilydue to its accuracy, speed and simplicity. However, point-of-care diagnosis is still not been tested withthe LAMP assay. We have developed a portable LAMP device for the monitoring of Leishmania infection. TheLAMP assay performed using our device can detect and amplify as little as 100 femtograms of L. donovaniDNA. In a preliminary study, we have shown that the device can also amplify L. donovani DNA present in VLand PKDL patient samples with high sensitivity (100%), specificity (98%) and accuracy (99%), and can beused both for diagnostic and prognostic analysis. To our knowledge, this is the first report to describe thedevelopment and application of a portable LAMP device which has the potential to evolve as a point-of-carediagnostic and prognostic tool for Leishmania infections in future.
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Journal of Biosciences
Volume 48, 2023
All articles
Continuous Article Publishing mode
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Journal of Biosciences | News
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To trigger further research on plant mitochondria, the Journal of Biosciences is bringing out a special issue titled "Plant Mitochondria: Properties and Interactions with Other Organelles".
Plant mitochondria are quite distinct and have unique features, such as a cyanide-insensitive alternate pathway. They also interact with chloroplasts to optimize photosynthetic carbon assimilation.
Submissions are welcome until 30 July 2023. The contributions can be original articles, short communications, reviews, or mini-reviews on any topic related to plant mitochondria.
Authors can submit their articles online at https://www.editorialmanager.com/jbsc/default2.aspx
Posted on April 12, 2023< -
Editorial Note on Continuous Article Publication
Posted on July 25, 2019Click here for Editorial Note on CAP Mode
Forthcoming Special issue.
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