• KANUPRIYA SINGH

      Articles written in Journal of Biosciences

    • IL-3 inhibits rat osteoclast differentiation induced by TNF-$\alpha$ and other pro-osteoclastogenic cytokines

      VIKRANT PIPRODE KANUPRIYA SINGH ANIL KUMAR SNEHAL R JOSHI MOHAN R WANI

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      IL-3, a haematopoiesis regulatory factor, has previously been shown to inhibit both mouse and humanosteoclast differentiation and bone resorption. Here, the role of rat IL-3 on rat osteoclast differentiation was evaluated to address whether the inhibitory action of IL-3 on osteoclastogenesis is conserved in variousspecies. It was observed that IL-3 inhibited rat osteoclast differentiation induced by both TNF-$\alpha$ and receptoractivator of NF-$\kappa$B ligand (RANKL). TNF-$\alpha$ is known to induce bone loss in postmenopausal osteoporoticwomen and it also synergise with many pro-osteoclastogenic cytokines to cause huge pathological bone loss.Importantly, it was found that rat IL-3 inhibits the synergistic action of TNF-$\alpha$ with RANKL and IL-1$\beta$, TGF$\beta$1and TGF-$\beta$3. IL-3 downregulates the TNF-$\alpha$-induced nuclear translocation of NF-$\kappa$B-p65 and c-fos withoutaffecting c-jun. Interestingly, we observed that IL-3 also inhibits osteoclast differentiation in vivo in rats induced by TNF-$\alpha$. All these results suggest that inhibitory action of IL-3 on osteoclastogenesis is conserved invarious species including mice, rats and humans. Thus, our results clearly indicate that IL-3 has therapeuticpotential to treat pathological bone loss in important skeletal diseases.

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