J R Kidwai
Articles written in Journal of Biosciences
Volume 1 Issue 4 December 1979 pp 401-407
Isolation and characterisation of cathepsin-B from bovine pancreas
A simple procedure for the isolation of cathepsin-B from bovine pancreas employing ammonium sulphate fractionation, DEAE cellulose chromatography and Sephadex G-200 gel filtration is described. The purified enzyme gave a single band on polyacrylamide gel electrophoresis. The molecular weight as determined by gel filtration of the enzyme was 26,850. Its
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