Hong-Yun Liu
Articles written in Journal of Biosciences
Volume 34 Issue 2 June 2009 pp 221-226 Articles
Selection of scFvs specific for the HepG2 cell line using ribosome display
Lei Zhou Wei-Ping Mao Juan Fen Hong-Yun Liu Chuan-Jing Wei Wen-Xiu Li Feng-Yun Zhou
The aim of this study was to construct a ribosome display library of single chain variable fragments (scFvs) associated with hepatocarcinoma and screen such a library for hepatocarcinoma-binding scFvs. mRNA was isolated from the spleens of mice immunized with hepatocellular carcinoma cell line HepG2. Heavy and k chain genes (VH and k) were amplified separately by RT-PCR, and an anti-HepG2 VH/k chain ribosome display library was constructed by assembling VH and k into the VH/k chain with a specially constructed linker by SOE-PCR. The VH/k chain library was transcribed and translated
Volume 45, 2020
All articles
Continuous Article Publishing mode
Click here for Editorial Note on CAP Mode
© 2021-2022 Indian Academy of Sciences, Bengaluru.