Articles written in Journal of Biosciences
Volume 44 Issue 6 December 2019 Article ID 0136 Article
To elucidate one mechanism by which putrescine (Put) functions in plant signaling under osmotic stress, Put and ABAcontents, and plasma membrane-NADPH oxidase (PM-NOX) activity were detected in wheat seedling leaves. Underosmotic stress, ABA and Put contents, PM-NOX activity, and PM-NOX-dependent O2.- production all increased. Theinhibitor tungstate (T) of ABA bio-synthesis reduced the increases in ABA and Put contents under osmotic stress. Theinhibitor D-arginine (D-Arg) of Put bio-synthesis didn’t reduce osmotic-induced increase of ABA, but it inhibited theincreases of PM-NOX activity and O2 . - production, and the inhibitory effects were reversed by exogenous Put. Thesefindings suggested that ABA might regulate Put biosynthesis, and Put might regulate PM-NOX activity. Treatments withthree inhibitors imidazole (I), diphenylene iodonium (DPI) and pyridine (P) of PM-NOX reduced significantly not only O2 . -production, but also the stress-induced increase of Put content, which indicated that O2 . - production might regulate Putbiosynthesis. Treatments with EGTA (Ca2+ chelator), La3+ and verapamil (V) (Ca2+ channel blockers) reduced significantlythe stress-induced increase of Put content, which suggested that Ca2+ might regulate Put biosynthesis. With thesefindings, it could be concluded that Put was involved in ABA signaling induced by osmotic stress via regulating PM-NOXactivity in wheat seedling leaves.
Volume 46 All articles Published: 25 March 2021 Article ID 0031 Article
The mitochondrion conformation and the contents of conjugated polyamines were investigated using theembryos of developing wheat (Triticum aestivum L.) grains of two cultivars differing in drought toleranceas experiment materials. After drought stress treatment for 7 days, the relative water content of embryoand relative increase rate of embryo dry weight of the drought-sensitive Yangmai No. 9 cv. decreased moresignificantly than those of the drought-tolerant Yumai No. 18 cv. Furthermore, the changes in mitochondrionconformation of Yangmai No. 9 were more marked. Meanwhile, the increases of the contents ofconjugated non-covalently spermidine (CNC-Spd) and conjugated covalently putrescine (CC-Put) of YumaiNo. 18 were more obvious than those of Yangmai No. 9. Treatment with exogenous Spd not onlyalleviated the injury of drought stress to Yangmai No. 9, but also enhanced the increase of CNC-Spdcontent and inhibited the change in the mitochondrion conformation of this cultivar. The treatments ofYumai No. 18 with two inhibitors, methylglyoxyl-bis (guanylhydrazone) and phenanthrolin, significantlyinhibited the drought stress-induced increases of CNC-Spd and CC-Put contents of the cultivar, respectively.Meanwhile, the treatments with the two inhibitors aggravated the injury of drought stress to YumaiNo. 18 and enhanced the change in the mitochondrion conformation of this cultivar. These results mentionedabove suggested that the CNC-Spd and CC-Put in embryo mitochondrion membrane isolated fromdeveloping grains could enhance the wheat tolerance to drought stress by maintaining the mitochondrionconformation.
Volume 46, 2021
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