Articles written in Journal of Biosciences
Volume 44 Issue 6 December 2019 Article ID 0126 Article
MicroRNAs have been reported to play a crucial role in ovarian cancer (OC) as the most lethal malignancy of the women.Here, we found miR-506-3p was significantly down-regulated in OC tissues compared with corresponding adjacent nontumortissues. Ectopic miR-506-3p expression inhibited OC cell growth and proliferation using MTT and colony formationassay. Additionally, flow cytometry analysis showed that the overexpression of miR-506-3p induced cell cycle G0/G1phase arrest and cell apoptosis in OC cells. A luciferase reporter assay confirmed that the myotubularin-related protein 6(MTMR6) was the target of miR-506-3p. The expression of MTMR6 was increased in OC tissues compared with adjacenttissues using immunohistochemistry. Elevated MTMR6 protein levels were confirmed in OC cells lines compared withimmortalized fallopian tube epithelial cell line FTE187 using western blotting. In addition, knockdown of MTMR6 imitatedthe effects of miR-506-3p on cell proliferation, cell cycle progression and apoptosis in OC cells. Furthermore, rescueexperiments using MTMR6 overexpression further verified that MTMR6 was a functional target of miR-506-3p. Our dataindicate that miR-506-3p might serve as a tumor suppressor gene and propose a new regulatory mechanism of MTMR6 bymiR-506-3p in OC.
Volume 45 All articles Published: 7 May 2020 Article ID 0067 Article
In this study, the regulatory effect of the overexpression of polarity protein Lgl2 on the nuclear export ofinfluenza A virus nucleoprotein in infected cells was investigated. A stable Tet-Off inducible MDCK cell lineexpressing a fusion protein comprising Lgl2 and an enhanced yellow fluorescent protein were used. TCID50analysis and neuraminidase activity analysis revealed that replication of influenza Avirus was inhibited in Lgl2overexpressing cells. By immunofluorescence microscopical observation at different time point post virusinfection, a retention of NP in cellular nucleus was found in Lgl2 overexpressing cells. Compared with normalMDCK cells, change in claudin-1 distribution between cell contacts caused by Lgl2 overexpression impairedthe barrier function of tight junction. These results suggest that changes in cell polarity induced by Lgl2overexpressing will affect virus NP transportation.
Volume 46, 2020
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