• G Krishnamoorthy

      Articles written in Journal of Biosciences

    • Histidine-15 and lytic activity of lysozyme

      Madhuri M Ugrankar G Krishnamoorthy Bala S Prabhananda

      More Details Abstract Fulltext PDF

      The literature data on the activity of histidine-15 modified hen egg white lysozyme are conflicting: the modified enzyme is reported to have more activity, similar activity or less activity by different authors. Amino acid analysis had shown modification of the single His-15. Detailed activity studies on His-15-modified (by iodoacetic acid or diethyl pyrocarbonate) lysozyme have shown that the contradicting reports are due to the specific choices of ionic strengths and cell wall substrate concentrations and can be attributed to the substrate being negatively charged. Our analysis suggests that even though histidine-15 is far removed from the active site of lysozyme, its chemical modification or binding of the negatively-charged substrate near it, changes the conformation around the active site. However, the change in the optimum activity on chemically modifying His-15 is small.

    • Fluorescence spectroscopy for revealing mechanisms in biology: Strengths and pitfalls


      More Details Abstract Fulltext PDF

      This article describes the basic principles of steady-state and time-resolved fluorescence. The formal equivalence of the twomethodologies is described first, followed by the extra advantages of time-resolved methods in revealing populationheterogeneity in complex systems encountered in biology. Several examples from the author’s work are described insupport of the above contention. Finally, several misinterpretations and pitfalls in the interpretation of fluorescence data andtheir remedy are described.

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