• GV KAKURINA

      Articles written in Journal of Biosciences

    • Relationship of intracellular proteolysis with CAP1 and cofilin1 in non-small-cell lung cancer

      E S KOLEGOVA GV KAKURINA E E SHASHOVA N V YUNUSOVA LV SPIRINA EA SIDENKO DN KOSTROMITSKIY A YU DOBRODEEV I V KONDAKOVA

      More Details Abstract Fulltext PDF

      The main cause of death in non-small-cell lung cancer (NSCLC) is tumor progression, in which metastasis andinvasion play an important role. The metastatic cascade is marked by a change in morphological, biological,biochemical and functional characteristics, including the acquisition of cellular mobility. The migration activityof tumor cells determines the work of actin-binding proteins that cause their functional partners CAP1 andcofilin. Of interest is the study of the regulation of working tandem CAP1/cofilin in NSCLC. The mechanismthat regulates the level of proteins in cells is proteolysis, carried out by proteasomes and calpains. Therefore,the aim of this study was to estimate the expression of CAP1/CFL1 mRNA and their protein level in NSCLCtissues, and to analyze the possible mechanisms of their regulation by the proteasome and calpain systems.Samples of NSCLC and histological unchanged lung tissue were used (n = 42). The CAP1 and CFL1 mRNAexpressions were determined by real-time PCR, the contents of proteins encoded by them were determined byWestern blotting, and the activity of proteasomes and calpains by the fluorimetric method. There was anincrease in the expression of mRNA and protein levels of CAP1 and cofilin in the tumor tissue compared withthe unchanged lung tissue. The expression of mRNA and the level of CAP1 in tumor tissue increased duringgrowth of the primary tumor. The cofilin level in the tumor tissue decreases against the background ofincreased expression of its mRNA. At the same time, during tumor growth, the activity of proteasomes andcalpains increased. A negative regression relationships between the activity of proteasomes and the levels ofCAP1 and cofilin, as well as the activity of calpains and the level of cofilin, were found. It can be assumed thatproteasomes and calpains are involved in the degradation of CAP1 and cofilin. The data obtained suggest theimportance of CAP1, cofilin and proteolytic systems in the tumor transformation and lymphogenousmetastasis.

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