• GAURAB GANGOPADHYAY

      Articles written in Journal of Biosciences

    • RNAi-mediated down-regulation of ITPK-2 enhanced inorganic phosphorus and minerals in the transgenic rice

      SHINJINI SENGUPTA SANANDA BHATTACHARYA ARITRA KARMAKAR SUBHRAJYOTI GHOSH SAILENDRA NATH SARKAR GAURAB GANGOPADHYAY KARABI DATTA SWAPAN K DATTA

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      Phytic acid or Myo-inositol hexakisphosphate is an essential compound for the rice plants. It remains in theform of phytate, a mixed salt of different mineral cations, in the seeds. The phytate breaks down duringgermination and provides the inorganic phosphorus and mineral ions to the seedlings. However, humans do notget the benefit of those essential ions from rice consumption due to the absence of phytase in the gut. Weenvisaged down-regulating ITPK, the gene behind the phytic acid biosynthesis so that its low amount wouldfacilitate a greater amount of free mineral ions in the endosperm. Since there are six homologues of rice ITPK,we studied their expression in seeds. Additionally, we undertook an in-silico analysis of the homologousproteins. Considering the results, we selected ITPK-2 for its RNAi-mediated embryo-specific down-regulationto obtain the low phytate rice. We obtained a 37% reduction of phytic acid content accompanied by a nearlythree-fold enhancement of inorganic phosphorus in the transgenic seeds. Additionally, the iron and zinc contentincreased in polished rice grains compared to the wild type. The results also showed that reduced phytic acidcontent did not affect the germination potential and seedling growth of the transgenic rice.

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