• Farhat A Khan

      Articles written in Journal of Biosciences

    • Isolation and characterization of lysosomal alpha-mannosidase of placental tissue

      Farhat A Khan Debkumar Basu

      More Details Abstract Fulltext PDF

      The acidic α-mannosidase was purified 4400-fold by affinity chromatography on Concavalin A-Sepharose and heat treatment at 65‡C in the presence of 1 mM zinc ion. The enzyme did not resolve into multiple forms as in the case of enzymes from human liver and human kidney. The pH optimum of the enzyme was 4.2 in citrate-phosphate buffer. The Km value for p-nitrophenyl-α-D-mannose was 1.9 mM. The molecular weight of the enzyme determined by gel filtration was 300,000. The enzyme contained 10.6% neutral sugars.

  • Journal of Biosciences | News

© 2017-2019 Indian Academy of Sciences, Bengaluru.