DURGADAS P KASBEKAR
Articles written in Journal of Biosciences
Volume 39 Issue 3 June 2014 pp 341-346
Volume 41 Issue 2 June 2016 pp 171-172 Clipboard
Volume 42 Issue 1 March 2017 pp 5-10 Commentary
Volume 42 Issue 1 March 2017 pp 15-21 Article
During ascogenesis in Neurospora, the ascospores are partitioned at the eight-nucleus stage that follows meiosis and apost-meiotic mitosis, and the ascospores that form in eight-spored asci are usually homokaryotic. We had previouslycreated novel TNt strains by introgressing four Neurospora crassa insertional translocations (EB4, IBj5, UK14-1, andB362i) into N. tetrasperma. We now show that crosses of all the TNt strains with single-mating-type derivatives of thestandard N. tetrasperma pseudohomothallic strain 85 (viz. TNta × 85A or TNtA × 85a) can produce rare eight-sporedasci that contain heterokaryotic ascospores, or ascospores with other unexpected genotypes. Our results suggest thatthese rare asci result from the interposition of additional mitoses between the post-meiotic mitosis and the partitioningof nuclei into ascospores, leading to the formation of supernumerary nuclei that then generate the heterokaryoticascospores. The rare asci probably represent a background level of ascus dysgenesis wherein the partitioning ofascospores becomes uncoupled from the post-meiotic mitosis. Ordinarily, the severest effect of such dysgenesis, theproduction of mating-type heterokaryons, would be suppressed by the N. crassa tol (tolerant) gene, thus explainingwhy such dysgenesis remained undetected thus far.
Volume 43 Issue 2 June 2018 pp 235-237 Series
The vertebrate inner nuclear membrane protein, lamin B receptor, has an N-terminal *200 residue nucleoplasmic domain(NTD), and a *420 residue C-terminal domain (CTD) that anchors the NTD to the INM. Chen et al (2016) showed theNTD interacts with Xist long noncoding RNA to effect X chromosome inactivation in female mammals. Tsai et al (2016)showed the CTD has sterol reductase activity that is essential for viability. And Nikolakaki et al (2017) proposed a model tointerconnect these disparate functions of this chimeric protein. It amuses me now to think back to 24 years ago, when I wasconcerned that these domains might have come together in a cloning artifact.
Volume 43 Issue 5 December 2018 pp 819-822 Series
Volume 44 Issue 2 June 2019 Article ID 0051 Series