• Chen Fang

      Articles written in Journal of Biosciences

    • Expression of a ribosome inactivating protein (curcin 2) inJatropha curcas is induced by stress

      Wei Qin Huang Ming-Xing Xu Ying Zhang Xin-Shen Chen Fang

      More Details Abstract Fulltext PDF

      The open reading frame (ORF) encoding curcin 2 was cloned from total genomic and cDNA ofJatropha curcas leaves, which were treated by drought, temperature stress and fungal infection, by polymerase chain reaction (PCR) and reverse transcriptase (RT)-PCR amplification. The ORF has 927 bp that encodes a precursor protein of 309 amino acid residues. There are high similarities with curcin and the conserved domain of ribosome inactivating proteins (RIPs). Antiserum to curcin recognized one band of 32 kDa on Western blot of the leaves treated by temperature stresses at 4°C and 50°C and by fungal infections ofPestalotia funerea, Curvularia lunata (Walk) Boed,Gibberelle zeae (Schw.) Petch. Two bands of 32 kDa and 65 kDa were recognized on Western blot of the leaves treated by 10%-40% polyethylene glycol (PEG). In addition, the 32 kDa band is nearly the molecular weight of curcin 2. This finding suggests that the protein of 32 kDa should be related to curcin 2. The presence of this protein molecular marker under stresses may provide an experimental foundation to study the stress proteins inJ. curcas.

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