• CHENGYU SHUI

      Articles written in Journal of Biosciences

    • Circ_0002711 knockdown suppresses cell growth and aerobic glycolysis by modulating miR-1244/ROCK1 axis in ovarian cancer

      WEIQUAN XIE LU LIU CHUANYONG HE MIN ZHAO RONG NI ZIXIONG ZHANG CHENGYU SHUI

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      It has been well investigated that circular RNAs (circRNAs) play important roles in various cancers. Thefunction of circ_0002711 and its underlying mechanisms in ovarian cancer (OC) remain unknown. qRT-PCRand western blot were performed to detect the expressions of circ_0002711, microRNA-1244 (miR-1244), andRho kinase 1 (ROCK1) in OC tissues and cells. MTT assay and colony formation assay were employed toevaluate cell proliferation. Detection of lactate production, glucose uptake, and ATP level and oxygen consumptionwere used to determine Warburg effect. Western blot was used to examine glycolysis or proliferationrelatedgenes. Dual-luciferase reporter assay and RIP pull down assay were used to address the relationshipamong circ_0002711, miR-1244, and ROCK1. In vivo tumor growth was evaluated in nude mice.Circ_0002711 was upregulated in OC tissues and cell lines. Circ_0002711 downregulation inhibited cellviability, colony formation ability and aerobic glycolysis. Circ_0002711 contained binding sites with miR-1244. Moreover, loss of miR-1244 undermined circ_0002711 downregulation-mediated function. ROCK1contained binding sites with miR-1244. MiR-1244 upregulation suppressed cell proliferation and aerobicglycolysis, which was rescued by enhanced expression of ROCK1. Circ_0002711 knockdown hamperedROCK1 expression by upregulating miR-1244 expression. Finally, decreased expression of circ_0002711inhibited tumor growth in vivo. Circ_0002711/miR-1244/ROCK1 axis regulated Warburg effect and tumorgrowth in vivo.

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