Articles written in Journal of Biosciences
Volume 44 Issue 4 September 2019 Article ID 0086 Article
Vascular calcification is a common problem in the elderly with diabetes, heart failure and end-stage renal disease. Thedifferentiation of vascular smooth muscle cells (VSMCs) into osteoblasts is the main feature, but the exact mechanismremains unclear. It is not clear whether adiponectin (APN) affects osteogenic differentiation of VSMCs. This study aims toexplore the effect of APN on vascular calcification by using a cell model induced by beta-glycerophosphate (b-GP).VSMCs were isolated and treated with b-GP and APN in this study. The alkaline phosphatase (ALP) activity andexpression levels of Runx2, BMP-2, collagen type I and osteocalcin were determined. The expression levels of STAT3 andp-STAT3 in nucleus and cytoplasm of VSMCs were analyzed. The results showed that APN significantly inhibited theexpression of ALP, Runx2, BMP-2, collagen I, osteocalcin and the formation of the mineralized matrix in VSMCs inducedby b-GP. APN reduces the osteogenic differentiation of VSMCs induced by b-GP and down-regulates the expression of theosteogenic transcription factor osterix by inhibiting STATS3 phosphorylation and nuclear transport. APN may be one of thepotential candidates for clinical treatment of vascular calcification.
Volume 45, 2020
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