• B V V Pardhasaradhi

      Articles written in Journal of Biosciences

    • Suppression of tumourogenicity and overexpression of cell adhesion molecules in AK-5 cells transfected with wild-type p53 gene

      Ashok Khar B V V Pardhasaradhi

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      We have previously shown overexpression of p53 and rearrangement of the p53 gene in AK-5 tumour. In order to study the role of p53 in AK-5 tumourogenicity, we introduced wild-type p53 in AK-5 cells. We have shown suppression of tumourogenicity in AK-5 cells after transfixion with wt p53. In one of the transfected clones 3B4, there was complete loss of tumour forming ability. Clone 3B4 also showed cellular aggregation which correlated well with the higher expression of cell adhesion molecules like fibronectin and hyaluronectin. These observations demonstrate tumour suppression in AK-5 after introduction of wt p53.

    • Differential cytotoxic effects ofAnnona squamosa seed extracts on human tumour cell lines: Role of reactive oxygen species and glutathione

      B V V Pardhasaradhi Madhurima Reddy A Mubarak Ali A Leela Kumari Ashok Khar

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      Annonaceous acetogenins are a new class of compounds that have been reported to have potent pesticidal, parasiticidal, anti-microbial, cell growth inhibitory activities. In this study, organic and aqueous extracts from the defatted seeds ofAnnona squamosa (custard apple) were tested on different human tumour cell lines for antitumoural activity. While organic and aqueous extracts induced apoptosis in MCF-7 and K-562 cells, they failed to do so in COLO-205 cells. Treatment of MCF-7 and K-562 cells with organic and aqueous extracts resulted in nuclear condensation, DNA fragmentation, induction of reactive oxygen species (ROS) generation and reduced intracellular glutathione levels. In addition downregulation of Bcl-2 and PS externalization by Annexin-V staining suggested induction of apoptosis in MCF-7 and K-562 cells by both the extracts through oxidative stress. On the contrary, COLO-205 cells showed only PS externalization but no change in ROS and glutathione levels. These observations suggest that the induction of apoptosis byA. squamosa extracts can be selective for certain types of cancerous cells

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