• B S Kundu

      Articles written in Journal of Biosciences

    • In vitro andin vivo nitrogenase activity ofRhizobium mutants and their symbiotic effectivity

      K R Dadarwal B S Kundu P Tauro

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      A slow growing nitrogen-fixing strain ofVigna radiata var.aureus (mung bean)Rhizobium which expressed nitrogenase activity in a synthetic medium was isolated from its native population. Mutants with decreased and increased nitrogenase activity were derived from this strain by treatment with acridine orange and ethidium bromide. These mutants were tested for symbiotic effectivity invivo. The effectivity of mutants with decreased nitrogenase activity in the culture medium was lower than the parent strain; however, the effectivity of mutants with higher nitrogenase activity did not increase above that of the parent. This suggests that the plant is perhaps a limiting factor in the full expression of rhizobial nitrogenase in the nodules.

    • Nitrification and simultaneous denitrification byAzospirillum brasilense 12S

      B S Kundu K R Dadarwal P Tauro

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      Azospirillum brasilense, an associative diazotrophs from sorghum roots grows autotrophically on NH4+ and CaCO3. NH4+ a is also oxidized to NO2- and then denitrified. Addition of malate to the autotrophic medium enhances both NH4+ oxidation as well as NONH2- dissimilation. The incomplete nitrification linked denitrification results in a rapid loss of nitrogen from the growth medium. The bacterium also shows assimilatory NO3- and NO2- reductases and fixes nitrogen at 50 μg N/ml of NH4+ NO3- or NO2-

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