• Asok K Bhattacharyya

      Articles written in Journal of Biosciences

    • Purification of human seminal acrosin inhibitor and its kinetics

      Aditi Chatterjee Sagarika Kanjilal Asok K Bhattacharyya

      More Details Abstract Fulltext PDF

      A low molecular mass, naturally occurring acrosin inhibitor has been identified and purified (490-7-fold) from human semen, and kinetic studies have been performed on the association characteristics as well as for the determination of affinity constants (Ki values). The results show thatKi value (3.34 × 10−2) of the inhibitor towards human acrosin is almost three times lower than that of pancreatic trypsin, indicating a much higher specificity and inhibitory property for acrosin. The purified human seminal acrosin inhibitor has a molecular mass of 5.5 kDa and shows a single band using 10–20% gradient SDS PAGE. The work is of great significance for the development of more specific, nontoxic and irreversible inhibitors for human acrosin.

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