Anil K Lala
Articles written in Journal of Biosciences
Volume 13 Issue 2 June 1988 pp 147-151
The yeast sterol auxotroph GL-7, which grows well on ergosterol and cholesterol, was used to study the ability of cholesteryl-(2′-hydroxy)-ethyl ether to substitute for cholesterol. In this compound the 3j3-hydroxyl group of cholesterol is replaced by ethylene glycol and the resulting ether still retains the amphiphilic character of cholesterol. Cholesteryl-(2Lhydroxy)-ethyl ether was found to support the growth of GL-7 as effectively as cholesterol. Crystal violet permeability and membrane order parameter determined using a spin label were similar for cells grown on these sterols. The ability of such ethylene glycol derivatives to substitute for cholesterol in both artificial and natural membranes should help in designing suitable spacers through which molecules can be linked to cholesterol without affecting the normal function of cholesterol in membranes. This in turn should prove useful in studies with surface-modified liposomes.
Volume 14 Issue 2 June 1989 pp 127-132
Synthesis of a radioactive photoactivable heterobifunctional reagent, N-oxysuccinimide ester of 2-[14C]glycyl carboxy-9-diazofluorene is described. This reagent on photolysis gives rise to a reactive carbene which rapidly inserts into solvents like methanol. The probe can be easily linked to aldolase which on photolysis gives rise to aldolase dimer, trimer and tetramer depending on the density of linked probe. This probe has also been linked to concanavalin A. The radioactive concanavalin A so obtained was incubated with erythrocyte ghosts and photolysed. The membrane protein analysis by gel electrophoresis indicated that concanavalin A has been covalently crosslinked to band 3.
Volume 24 Issue S1 March 1999 pp 5-31
F Parak A Ostermann G U Nienhaus Nobuo Niimura William A Eaton Stephen J Hagen Eric R Henry James Hofrichter Gouri Jas Lisa Lapidus Victor Muñoz Chih-chen Wang Abani Bhuyan Javant Udgaonkar Heinz Rüterians Derek N Woolfson M D Finucane J H Lees M J Pandya G Spooner M Tuna Wilma K Olson K V R Chary E Westhof I G Wool C C Correll V I Ivanov S A Bondarenko E M Zdobnov A D Beniaminov E E Minyat N B Ulyanov Dale B Wigley Nobuo Shimamoto Takashi Kinebuchi Hiroyuki Kabata Osamu Kurosawa Masao Washizu Barbara Baird David Holowka H Belrhali P Nollert A Royant J P Rosenbusch E M Landau E Pebav-Peyroula Anil K Lala Patrick R D’Silva Daniela Pietrobon Paolo Pinton Paulo Magalhaes Anna Chiesa Marisa Brini Tullio Pozzan Rosario Rizzuto M Montai Shu-Rong Wang José L Carrascosa B Bhattacharyya Ian A Wilson Dinakar M Salunke Kurt Drickamer Anne Imberty A Surolia Louise N Johnson Michal Neeman S M Prince K McLuskey R J Cogdell K McAuley N W Isaacs G Venturoli F Drepper J C Williams J P Allen X Lin P Mathis R van Grondelle Wolfgang Junge T Tsukihara K Shinzawa-Itoh R Nakashima E Yamashita M J Fei N Inoue T Tomizaki C Peters Libeu S Yoshikawa Patrick Chaussepied Keiichi Namba Marie-France Carlier Fariza Ressacl Valerie Laurent Thomas Loisel Coumaran Egile Philippe Sansonetti Dominique Pantaloni Manju Bansal E W Knapp M G Ullmann A Amadei B L de Groot M A Ceruso M Paci H J C Berendsen A Di Nola V Di Francesco P J Munson J Garnier Sung-Hou Kim Jean-Michel Claverie Ian C P Smith P T Callaghan Bruce Cornell Ratna S Phadke Kazuhiko Kinosita D Goldfarb I Qromov C Shutter I Pecht P Manikandan R Carmieli T Shane David S Moss Clare E Sansom Jeremy K Cockcroft Ian J Tickle Huub C P Driessen J Raul Grigera Ramen K Poddar Charles R Cantor Barry Robson Jean Garnier John Helliwell Sunney I Chan Ronald Rock