Anandwardhan A Hardikar
Articles written in Journal of Biosciences
Volume 24 Issue 3 September 1999 pp 371-376 Articles
Techniques for immunoisolation and immobilization of viable cells within semipermeable microcapsules have been developed using highly sophisticated droplet generator systems. We propose here an indigenously designed, simple and efficient droplet generator system for encapsulation of the pancreatic islets employing chitosanalginate matrix. The droplet generator system comprises of a needle assembly, a 3-way valve with extended rubber tubing and a filtration unit connected to a pressure pump. Microbeads of the size of around 400 μm diameter or even lesser (minimun attainable size 20.2 μm) could be easily generated using the droplet generator system proposed here. Islet microcapsules cultured in Roswell Park Memorial Institute (RPMI) 1640 with 10% fetal calf serum showed around 98% viability, comparable to that of the non-encapsulated islets. Transplantation of microencapsulated islets to streptozotocin (STZ)-induced diabetic mice, resulted in disappearance of hyperglycemia and restoration of normoglycaemia during a 30-day follow-up suggesting graft functionality. No graft failures were observed in any of the transplanted mice (
Volume 34 Issue 4 October 2009 pp 523-528 Articles
Phenotypic plasticity is a phenomenon that describes the occurrence of 2 or more distinct phenotypes under diverse conditions. This article discusses the work carried out over the past few years in understanding the potential of human pancreatic islet-derived progenitors for cell replacement therapy in diabetes. The phenotypic plasticity exhibited by pancreatic progenitors during reversible epithelial-to-mesenchymal transition (EMT) and possible role of microRNAs in regulation of this process is also presented herein.
Volume 44 | Issue 5
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