Articles written in Journal of Biosciences
Volume 15 Issue 3 September 1990 pp 143-144
Membrane penetration depth is an important parameter in relation to membrane structure and organization. A methodology has been developed to analyze the membrane penetration depths of fluorescent molecules or groups utilizing differential fluorescence quenching caused by membrane embedded spin-label probes located at different depths. The method involves determination of the parallax in the apparent location of fluorophores, detected when quenching by phospholipids spin-labelled at two different depths is compared. By use of relatively simple algebraic expressions, the method allows calculation of depth in å. This method has been used to determine the location of fluorophores in NBD-labelled lipids and anthroyloxy-labelled fatty acids in model membranes and of the membrane embedded tryptophan residues in the reconstituted nicotinic acetylcholine receptor.
Volume 29 Issue 1 March 2004 pp 1-2 Clipboard
Volume 30 Issue 2 March 2005 pp 147-149 Commentary
Volume 31 Issue 3 September 2006 pp 297-302 Commentary
Volume 34 Issue 2 June 2009 pp 169-172
Volume 43 Issue 3 July 2018 pp 421-430 Commentary
Use of Green Fluorescent Protein (GFP) as a marker has revolutionized biological research in the last few decades. In this briefcommentary, we reflect upon the success story of GFP and highlight a few lesser-known facets about GFP that add up to itsusefulness.