Articles written in Journal of Biosciences
Volume 11 Issue 1-4 March 1987 pp 257-263
The products generated from linoleic acid by the two forms of Bengal gram lipoxygenase, BGL1 and BGL2, were analysed by high-performance liquid chromatography using μ-porasil column with isooctane containing 0.5% ethanol as the solvent system. The l3-hydroperoxyoctadecadienoic acid and its 9-isomer which are known to be produced by soybean lipoxygenase-1 and the potato enzyme respectively were used as standards. The results show that BGL1 generated almost exclusively the l3-hydroperoxyoctadecadienoic acid while BGL2 produced both l3-and the 9-isomer in the ratio 21:79. The secondary keto derivatives formed in the BGL2 reaction were also separated by this technique.
Volume 46, 2021
Continuous Article Publishing mode
Click here for Editorial Note on CAP Mode