• Adnan Khan

      Articles written in Journal of Biosciences

    • Development of rapid phenotypic system for the identification of Gram-negative oxidase-positive bacilli in resource-limited settings

      Mahmooda Kazmi Adnan Khan Shahana Urooj Kazmi

      More Details Abstract Fulltext PDF

      Rapid and accurate identification of bacterial pathogens is a fundamental goal of clinical microbiology. The diagnosis and surveillance of diseases is dependent, to a great extent, on laboratory services, which cannot function without effective reliable reagents and diagnostics. Despite the advancement in microbiology diagnosis globally, resource-limited countries still struggle to provide an acceptable diagnosis quality which helps in clinical disease management and improve their mortality and morbidity data. During this study an indigenous product, Quick Test Strip (QTS) NE, was developed for the rapid identification of biochemically slower group of Gram-negative oxidase-positive bacilli that covers 19 different bacterial genera. Some of the members belonging to these groups are well-established human pathogens, e.g. various species of Vibrio, Pseudomonas, Burkholderia, Aeromonas, Achromobacter and Stenotrophomonas. This study also evaluates the performance of QTS-NE by comparing with genotypic characterization methods. A total of 232 clinical and reference bacterial isolates were tested by three different methods. QTS-NE provides 100% concordant results with other rapid identification and molecular characterization methods and confirms the potential to be used in clinical diagnosis.

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