• ANNIE JOHN

      Articles written in Journal of Biosciences

    • Hormonal modulation of glycogen reserves In the fat body of castor semilooperAchaea janata Linn (Lepidoptera, Noctuidea)

      Annie John D Muraleedharan

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      Histochemical details of the fat body in the fifth instar larval stage, pupa and adult moth of the castor semilooperAchaea janata were elucidated in detail using light and electron microscopy in conjunction with glycogen storage patterns using polyacrylamide gel electrophoresis. The periodic-acid Schiff staining for glycogen in fat body was maximum in the spinning stage of the larva, when compared to the feeding stage and prepupal stages, and higher in the pupa than in the larva and the adult moth. In insulin injected and juvenile hormone treated fat body, glycogen deposition was more than in glucagon injected tissues. The periodic-acid Schiff stained bands in PAGE had electrophoretic mobility similar to the corresponding protein band numbers, indicating their glycoprotein nature.

    • Green synthesis of Cuminum cyminum silver nanoparticles: Characterizations and cytocompatibility with lapine primary tenocytes

      D S AMRUTHA JOSNA JOSEPH CA VINEETH ANNIE JOHN ANNIE ABRAHAM

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      Current treatment systems for tendon injuries are very few and do not ensure complete cure. This is a serioushealth concern for sports persons and the aged population. It is known that the nano- or microsized particles ofnatural products such as jeera/cumin seed (Cuminum cyminum) has been used traditionally as a home remedyfor the treatment of tendon injuries. Nevertheless, these particles are likely to perform better due to theirsmaller size, increased absorption and local delivery in conjunction with nanotechnology. In this context, themajor objective of this study was to synthesize silver-capped nanoparticles using aqueous extract of Cuminumcyminum (CCE) and to assess their in vitro non-cytotoxic effect with the perspective of clinical application toenhance tendon tissue regeneration. The presence of phytochemicals in CCE was studied by qualitative andquantitative methods. Cuminum cyminum nanoparticles (CCNP) were synthesized by the bioreduction methodusing silver nitrate and the particles were characterized by X-ray diffraction analysis (XRD), Fourier TransformInfra Red Spectroscopy (FTIR), Zeta potential measurement and scanning electron microscopy (SEM). Theantioxidant effect of the particles was studied using total antioxidant activity and reducing power assay.Simultaneously, primary Tenocytes were isolated from rabbit Achilles tendon by collagenase and dispasedigestion/treatment and characterized for Type 1 collagen. Further, in vitro non-cytotoxicity of the CCNP indirect contact with L929 mouse fibroblast cells and primary Tenocytes, respectively, was evaluated by MTTassay. Physico-chemical characterizations confirmed the formation and stability of the nanosize of CCNP withantioxidant property. Again, MTT assay confirmed the non-cytotoxicity of CCNP with L929 fibroblasts andprimary Tenocytes. CCNP may be attributed as an ideal candidate for therapeutic application towards a fasterrestoration of worn-out/injured tendon tissue confronted by the geriatric and athlete communities.

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