• ANJU SHRIVASTAVA

      Articles written in Journal of Biosciences

    • Deletion of Dictyostelium discoideum Sir2A impairs cell proliferation and inhibits autophagy

      RAKHEE LOHIA PUNITA JAIN MUKUL JAIN HIMANSHU MISHRA PRADEEP KUMAR BURMA ANJU SHRIVASTAVA SHWETA SARAN

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      Sirtuins are a family of deacetylases (Class III histone deacetylases) with evolutionarily conserved functions in cellularmetabolism and chromatin regulation. Out of the seven human Sirtuins, the function of Sirt2 is the least understood. Thepurpose of the present study was to investigate the role of Sir2A, a homolog of human Sirt2 in Dictyostelium discoideum(Dd), a lower eukaryote. We created both overexpressing and deletion strains of Ddsir2A to analyse its functions. Weobserved sir2A mRNA expression throughout development and the transcript was present in the prespore/spore region ofmulticellular structures developed. They show a preference towards prestalk/stalk pathway when co-developed with wildtypecells during chimera formation. Deletion strain showed a multi-tipped phenotype, decrease in cell proliferation andinhibition of autophagy. In conclusion, our results show low cAMP levels, reduced cell-adhesion, weak cell migration andimpaired autophagy to be responsible for the phenotype shown by the null cells. This study provides new insights into thefunctions of Ddsir2A.

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