Articles written in Journal of Biosciences

    • Fluorescence-based approaches for monitoring membrane receptor oligomerization


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      Membrane protein structures are highly under-represented relative to water-soluble protein structures in the protein databank.This is especially the case because membrane proteins represent more than 30% of proteins encoded in the humangenome yet contribute to less than 10% of currently known structures (Torres et al. in Trends Biol Sci 28:137–144, 2003).Obtaining high-resolution structures of membrane proteins by traditional methods such as NMR and x-ray crystallographyis challenging, because membrane proteins are difficult to solubilise, purify and crystallize. Consequently, development ofmethods to examine protein structure in situ is highly desirable. Fluorescence is highly sensitive to protein structure anddynamics (Lakowicz in Principles of fluorescence spectroscopy, Springer, New York, 2007). This is mainly because of thetime a fluorescence probe molecule spends in the excited state. Judicious choice and placement of fluorescentmolecule(s) within a protein(s) enables the experimentalist to obtain information at a specific site(s) in the protein (complex)of interest. Moreover, the inherent multi-dimensional nature of fluorescence signals across wavelength, orientation, spaceand time enables the design of experiments that give direct information on protein structure and dynamics in a biologicalsetting. The purpose of this review is to introduce the reader to approaches to determine oligomeric state or quaternarystructure at the cell membrane surface with the ultimate goal of linking the oligomeric state to the biological function. In thefirst section, we present a brief overview of available methods for determining oligomeric state and compare theiradvantages and disadvantages. In the second section, we highlight some of the methods developed in our laboratory toaddress contemporary questions in membrane protein oligomerization. In the third section, we outline our approach todetermine the link between protein oligomerization and biological activity.

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      Forthcoming Special issue.

    • To trigger further research on plant mitochondria, the Journal of Biosciences is bringing out a special issue titled "Plant Mitochondria: Properties and Interactions with Other Organelles".

      Plant mitochondria are quite distinct and have unique features, such as a cyanide-insensitive alternate pathway. They also interact with chloroplasts to optimize photosynthetic carbon assimilation.

      Submissions are welcome until 30 July 2023. The contributions can be original articles, short communications, reviews, or mini-reviews on any topic related to plant mitochondria.

      Authors can submit their articles online at https://www.editorialmanager.com/jbsc/default2.aspx

      Posted on April 12, 2023
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      Posted on July 25, 2019

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