Articles written in Journal of Biosciences
Volume 42 Issue 3 September 2017 pp 427-438 Article
Leishmania establishes a successful parasitism by evading both oxidative and non-oxidative killing pathways, and its drugresistance against the currently available therapeutics demands for a safe and cheap drug. Since the parasite synthesizesergosterol instead of cholesterol, using the same biochemical pathway and enzymes, an inhibitor of HMG-CoA-Reductase,Lovastatin, has been tried for its anti-Leishmanial effect. Lovastatin, being an inhibitor of HMG-CoA-Reductase, inhibitsinfection by cholesterol depletion, while chromium chloride complexes, at their higher concentrations, are reported toexhibit cytotoxicity. In intracellular amastigotes, cytotoxicity has been checked by assessing various manifestation of celldeath, viz. DNA fragmentation, AnnexinV-FITC binding and JC-1 fluorescence ratio. Release of hydrogen peroxide (HPO)and nitric oxide (NO) has been assessed in live cell. Lovastatin and CrCl3.6H2O in combination has appeared to beineffective on promastigotes but has induced cytotoxic effect on the intracellular amastigotes through up-regulation ofcellular signalling mechanisms. CrCl3.6H2O stimulates generation of NO, leading to reduction of the number of intracellularamastigote, while Lovastatin shows HPO-mediated killing of the same, keeping the host cell unaffected. This noveltherapeutic approach, involving two known safe compounds in suboptimal doses, may resolve human visceralLeishmaniasis.
Volume 44 Issue 4 September 2019 Article ID 0089 Article
Constantly rising energy demands, finite fossil fuel reserves and deteriorating environmental conditions have invokedworldwide interest to explore the sustainable sources of renewable biofuels. Locally adapted photosynthetic oleaginousmicroalgae with rapid growth on variable temperatures could be an ideal way for bioremediating the wastewater (WW) whileproducing the feedstock for biodiesel. To test this notion, an unknown strain was isolated from a sewage fed lake (Neela-Hauz).It was discerned as Chlorella sorokiniana-I using the 16S rDNA and 18S rDNA barcodes. The culture conditions such as pH,illumination, different temperature ranges and growth medium were cohesively optimized prior to the assessment of C.sorokiniana-I’s efficacy to remediate the WW and biodiesel production. The strain has thrived well up to 40 deg C when continuouslygrown for 15 days. The highest lipid accumulation and biomass productivity were recorded in 100%WW. Fatty acidmethyl ester (FAME) content was observed to be more than twice in WW (47%), compared to control synthetic media, TAP(20%) and BG11 (10%), which indicate the importance of this new isolate for producing economically viable biodiesel.Moreover, it is highly efficient in removing the total nitrogen (77%), total phosphorous (81%), iron (67%) and calcium (42%)from the WW. The quality of WW was considerably improved by reducing the overall chemical oxygen demand (48%),biological oxygen demand (47%) and alkalinity (15%). Thus, C. sorokiniana-I could be an ideal alga for the tropical countriesin the remediation of WW while producing feedstock for biodiesel in a cost-effective manner.
Volume 45 All articles Published: 24 November 2020 Article ID 0148 Review
An unprecedented worldwide spread of the SARS-CoV-2 has imposed severe challenges on healthcarefacilities and medical infrastructure. The global research community faces urgent calls for the development ofrapid diagnostic tools, effective treatment protocols, and most importantly, vaccines against the pathogen.Pooling together expertise across broad domains to innovate effective solutions is the need of the hour. Withthese requirements in mind, in this review, we provide detailed critical accounts on the leading efforts atdeveloping diagnostics tools, therapeutic agents, and vaccine candidates. Importantly, we furnish the readerwith a multidisciplinary perspective on how conventional methods like serology and RT-PCR, as well ascutting-edge technologies like CRISPR/Cas and artificial intelligence/machine learning, are being employed toinform and guide such investigations. We expect this narrative to serve a broad audience of both active andaspiring researchers in the field of biomedical sciences and engineering and help inspire radical newapproaches towards effective detection, treatment, and prevention of this global pandemic.
Volume 46 All articles Published: 19 May 2021 Article ID 0044 Review
Volume 46, 2021
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