• AKBAR KARKHANEH

      Articles written in Journal of Biosciences

    • Effect of PEDOT:PSS in tissue engineering composite scaffold on improvement and maintenance of endothelial cell function

      MASOUMEH HESAM MAHMOUDINEZHAD AKBAR KARKHANEH KHOSROW JADIDI

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      According to recent research, smart polymers can affect different kind of mammalian cells such as endothelial cells. It isknown that conductive polymers have great features, e.g. electrical conductivity, and can help increase electrical cellcommunication. To clarify the effect of one of these smart materials on endothelial cells, which are not inherentlyelectrically dependent, poly(3, 4-ethylene dioxythiophene) polystyrene sulfonate (PEDOT:PSS) was chosen. Scaffolds werecomposed of gelatin, alginate, and PEDOT:PSS and made through solvent casting. Human umbilical vein endothelial cells(HUVECs) were cultured on the scaffold with different PEDOT:PSS concentrations. SEM, MTT assay, cell attachment,nitric oxide measurement, real-time PCR and immunohistochemistry analysis were employed to assess endothelial cellresponses. Although there was no significant difference in swelling ratio, mass loss, and cell attachment when PEDOT:PSSconcentration increased in scaffold construction, cell proliferation noticeably increased after seven days. The cells showed asignificant increase in proliferation and NO release to the scaffold with 1% PEDOT:PSS concentration. The resultsindicated increases in the amount of expression of platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31), kinaseinsert domain receptor (KDR), vascular-endothelial Cadherin (VE. Cadherin), and von Will brand factor (vWf) in the groupwhich contained a conductive polymer in comparison with the non-conductive scaffold. Therefore, as a conductivepolymer, PEDOT:PSS can affect the endothelial cell behaviours.

    • On-demand release of ciprofloxacin from a smart nanofiber depot with acoustic stimulus

      SAJEDEH KHORSHIDI AKBAR KARKHANEH

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      Herein, an antibiotic-loaded electrospun scaffold with improved drug delivery via acoustic stimulation has been developed.Ultrasound stimulus with an intensity of 15 W/cm2, duty-cycle of 50% and duration of 10 min was repeatedly applied tociprofloxacin loaded alginate fibers. Ultrasonication with the aforesaid conditions increased drug release from scaffoldprobably due to disturbance of ionic crosslinks of alginate network. Scaffolds exposed to acoustic stimulus revealed higherantibacterial activity compared to those with no stimulus. Interestingly, antibiotic release radically increased andantibacterial function improved as ultrasound perturbed scaffold framework, but scaffold integrity was regained once theultrasound probe was retracted.

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