• AA SULTAN

      Articles written in Journal of Biosciences

    • Antifolate drug resistance: Novel mutations and haplotype distribution in dhps and dhfr from Northeast India

      NP SARMAH K SARMA DR BHATTACHARYYA AA SULTAN D BANSAL N SINGH PK BHARTI R SEHGAL PK MOHAPATRA P PARIDA J MAHANTA

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      Malaria is a major public health concern in Northeast India with a preponderance of drug-resistant strains. Until recently thepartner drug for artemisinin combination therapy (ACT) was sulphadoxine pyrimethamine (SP). Antifolate drug resistancehas been associated with the mutations at dihydropteroate synthase (dhps) and dihydrofolatereductase (dhfr) genes. Thisstudy investigated antifolate drug resistance at the molecular level. A total of 249 fever cases from Arunachal Pradesh, NEIndia, were screened for malaria, and of these, 75 were found to be positive for Plasmodium falciparum. Samples weresequenced and analysed with the help of BioEdit and ClustalW. Three novel point mutations were found in the dhps genewith 10 haplotypes along with the already reported mutations. A single haplotype having quadruple mutation was found inthe dhfr gene. The study reports higher degree of antifolate drug resistance as evidenced by the presence of multiple pointmutations in dhps and dhfr genes. The findings of this study strongly discourage the use SP as a partner drug in ACT.

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