A H Bandivdekar
Articles written in Journal of Biosciences
Volume 7 Issue 2 March 1985 pp 175-190
Isolation of inhibin like peptides from human placenta
A H Bandivdekar S B Moodbidri A R Sheth
Two moieties of inhibin could be obtained by chromatography of partially purified preparations of inhibin from human placenta on Sephadex G-100, G-25 and ion exchange chromatography on diethylaminoethyl Sephadex A-50. The higher molecular weight moiety (14,000) designated as HPI-H appears to be similar to inhibin from human seminal plasma. While the lower molecular weight moiety (1500) designated as HPI-L appears to be similar to that of sheep testicular inhibin.
The preparations from both human term placenta and human seminal plasma inhibited the binding of [125I] human follicle stimulating hormone to rat testicular receptors. This effect of inhibins could be neutralized by antisera raised against corresponding polypeptide. Further these antibodies could neutralize endogenous inhibin resulting in 2 to 3 fold increase in serum follicle stimulating harmone levels, which could then be reversed by exogenous administration of the isolated inhibin preparations.
Volume 48, 2023
Continuous Article Publishing mode
Click here for Editorial Note on CAP Mode