In this study, bionanocatalysts composed of a titanosilicate oxide matrix (TiO$_2$–SiO$_2$) impregnated with different concentrations of silver (0.1, 0.5, 1.0, 5.0 and 10 wt%) were prepared by the sol–gel method using silver nitrate(AgNO$_3$) as silver (Ag) source. The physicochemical properties were evaluated by scanning and transmission electron microscopies, Raman and Fourier transform infrared spectra, X-ray photoelectron spectra, and UV–visible spectroscopy.Furthermore, we studied the cytotoxic effect on a cervical uterine cancer cell line as a first approach attending to the potential antineoplastic properties of the Ag/TiO$_2$–SiO$_2$ bionanocatalysts. The bionanocatalysts exhibited no significant differences in morphology or composition independently of the amount of Ag stabilized, with a reduction in particle size proportional to Ag concentration. All samples could achieve cell mortality rates above 91%, obtaining results similar tothose of pure AgNO$_3$ (considered as 100% Ag) from the sample loaded with 1% Ag. The results in this study suggest the possibility of using bionanocatalysts to enhance the intrinsic cytotoxic properties of silver such that a minimum concentration is required to achieve desired results.