• NASIM MALEKI

      Articles written in Bulletin of Materials Science

    • Laccase immobilized onto graphene oxide nanosheets and electrodeposited gold–cetyltrimethylammonium bromide complex to fabricate a novel catechol biosensor

      MARYAM NAZARI SOHEILA KASHANIAN NASIM MALEKI NAHID SHAHABADI

      More Details Abstract Fulltext PDF

      In this study, a new biosensor is developed with reliable and easy-to-use biodevice properties for catechol determination in real samples. A method is proposed for the fabrication of biosensors to sense catechol based on theadsorption method of laccase immobilization. Hence, a glassy carbon electrode was modified via graphene oxide nanosheets and then it was modified with a gold–cetyltrimethylammonium bromide nanocomposite to adsorb and immobilize laccase on the electrode surface. The results showed laccase immobilization onto the reformed glassy carbon electrode, and a direct electron transfer reaction between laccase and the electrode. The mechanism of electron transferring was EC$^{\prime}$. Also, $k_s$ and $\alpha$ were calculated as 0.41 s$^{−1}$ and 0.33, respectively. For this biosensor two linear ranges, $0.1 \times 10^{−6}$ to $5 \times 10^{−6}$ M and $16.7\times 10^{−6}$ to $166 \times 10^{−6}$ M, and a detection limit of $1.5 \times 10^{−6}$ M were obtained.

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